Enhanced Reactive Oxygen Species (ROS) Assay Kit

Description

Enhanced Reactive Oxygen Assay Kit is a kit that utilizes the fluorescent probe H2DCF-DA for the detection of reactive oxygen species. The probe H2DCF-DA itself is non-fluorescent and can freely pass through the cell membrane.Detection type:Cell-based (quantitative);Sample Type:cells ( including adherent cells and suspension cells );Detection Method:Fluorescent;Detection Instrument:Fluorescence microplate reader (measure the fluorescence value at 488 nm excitation wavelength and 525 nm emission wavelength. ) Flow cytometer (set the excitation wavelength to 488 nm and the detection wavelength to 525 nm. The fluorescence spectrum of DCF is very similar to that of FITC. The parameters of FITC can be used to detect DCF. The number of cells can be selected to be 104-105 during detection ) Laser confocal microscopy