eSpCas9 mRNA(Cap1, m1Ψ)

Description

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al Science. 2015 Dec 1. eSpCas9 can reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100 % substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.