Exonuclease I

Description

E. coliExonuclease I is a 35 exonuclease specific for single-stranded DNA, which results in the production of 5-phosphate mononucleotides. This enzyme has a strict specificity for single-stranded DNA and does not react with double-stranded DNA or RNA. It is inactivated by heat treatment at 80C for 15 minutes. Cat. # 2650B contains 5 of Cat. # 2650A. Please refer to Cat. # 2650A for complete product documentation and resources. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back E.coliExonuclease I is a 35 exonuclease specific for single-stranded DNA. This enzyme does not digest double-stranded DNA or RNA. To demonstrate this specificity, single-stranded DNA ssDNA, 100-mer and a double-stranded DNA fragment from pBR322 were incubated with different amounts of Exonuclease I 1050 units . The digestion products were then analyzed on an agarose gel. Reactions were incubated at 37C for 30 minutes. Results:The ssDNA was digested by Exonuclease I, but the pBR322 fragment remained intact. Conclusion:Exonuclease I digests only ssDNA. Back