

2480B · 2,000 U
Takara · Cat: 2480B
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takarabio.com/products/mrna-and-cdna-synthesis/in-vitro-tran...
Description
Faustovirus Capping Enzyme S17 adds 7-methylguanylate cap structures Cap-0 to the 5 ends of uncapped RNA. Faustovirus Capping Enzyme 25 U/mul , 10X Capping Buffer 2, S-adenosylmethionine SAM , and GTP are included. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Effect of temperature on capping efficiency of FCE and VCE.Capping reactions were performed using Faustovirus Capping Enzyme S17 FCE; Cat. # 2480 or Vaccinia Capping Enzyme VCE; Cat. # 2460 at various reaction temperatures. The capping efficiency % Cap-0 RNA was measured by LC-MS. FCE demonstrated increased capping efficiency over a broader temperature range than VCE. Back







