Flag-Tag Rapid Competitive Assay Kit

Description

Flag-Tag Rapid Competitive Assay Kit adopts colloidal gold lateral flow technology based on competitive immunoassay. Recombinant Flag-Tag protein is immobilized on the test line of chromatography membrane, and anti-secondary antibody is fixed on the control line; colloidal gold-labeled anti-Flag-Tag monoclonal antibody is coated on the conjugate pad. In the absence of Flag-Tag protein in samples, gold-labeled antibodies migrate freely to the test line and bind with immobilized Flag protein to form distinct purplish-red bands. When Flag-Tag protein exists in samples, target proteins preferentially combine with gold-labeled antibodies to occupy binding sites and block antibody binding to test line antigens. Higher Flag-Tag concentration leads to fainter test line; the test line disappears completely above 10μg/mL. The limit of detection is 1μg/mL, and band intensity is negatively correlated with protein concentration ranging from 1μg/mL to 10μg/mL.