FLUID LACTOSE MEDIUM

Description

Coliforms are rod shaped gram-negative organisms that ferment lactose with the production of acid and gas. They are regarded as bacterial indicators of sanitary quality of foods and water. Salmonella is a rod shaped gram-negative enterobacteria commonly implicated in foodborne illness. These bacteria are present in low numbers in food and other products and also may be in a stressed condition. Before subjecting them to selective enrichment, for maximum recovery a pre-enrichment is necessary. Also, the presence of non-coliform bacteria and substances indigenous to the sample may interfere with the growth and recovery of coliforms. Therefore, pre-enrichment in a non-selective medium facilitates detection of sub-lethally injured cells. Fluid Lactose Medium is a pre-enrichment medium, recommended by APHA, for the detection of coliform bacteria in water, dairy products and food samples. When competing lactose utilizing bacteria are present in the test sample, a resulting drop in pH generates a bacteriostatic effect on the competing microflora. It is also used in the performance of test for Salmonella species and Escherichia coli.