FLUOR DE LYS-Succinyl, Desuccinylase Substrate

Description

The FLUOR DE LYSSuccinyl, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYSSuccinyl, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc. . Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYSDeveloper Prod