β-Gal Senescence Staining Kit

Description

Cell senescence refers to the gradual decline in cell proliferation, differentiation capacity, and physiological functions that occur over time during life activities. Under normal circumstances, the body eliminates senescent cells, while those remaining may accumulate, potentially causing low-level inflammation or further inducing tissue degeneration and carcinogenesis. Key characteristics of cellular senescence include morphological changes, cell cycle arrest, senescence-associated secretory phenotype, macromolecular damage, and metabolic disorders. Currently, the most specific marker for identifying senescence is senescence-associated β-galactosidase (SA-β-Gal), which accumulates with cellular aging and is absent in pre-senescence cells, quiescent cells, and terminally differentiated cells. This kit utilizes the fact that β-Gal in senescent cells can hydrolyze 5-bromo-4-chloro-3-indolyl-β-D-pentose galactoside (X-gal) into a blue substance at pH 6.0, enabling observation of cellular or tissue senescence under optical microscopy.