gamma-secretase Deficient E-Cre-ERT2 Stable Expressing Rat1 (AD6 Clone 1) Cell Line

Description

This rat cell line expresses a chimeric molecule containing a delta E-like fragment of Notch1 fused to a vesicular stomatitis virus epitope the Cre recombinase and a modified hormone binding domain of the estrogen receptor. The delivery of Cre recombinase depends on any event including gamma-secretase processing that releases the intracellular domain of Notch1 from the membrane. Nuclear activity of the Cre-ER-T2 chimera depends on the presence of tamoxifen. This cell line was derived from clones deficient in gamma-secretase activity thus displaying impaired activity on Notch. Reporter construct for the recombinase activity consists of an antibiotic resistance gene hygromycin . This cell line would be well-suited for research regarding the functions of the Notch receptor gamma-secretase enzyme activity and their involvement in cellular signalling.