B.A.G.G BROTH BASE (BUFFERED AZIDE GLUCOSE GLYCEROL BROTH BASE)

Description

Enterococci are commensals of the gut and are low-grade pathogens. However, in rare cases they cause urinary tract infections in catheterized patients, abdominal wound infections following gut surgery and endocarditis. Hajna and Perry developed Streptococcus faecalis Broth for the detection of faecal Streptococci, in water, milk and other materials. SF Broth is used for identification of Enterococci based on carbohydrate fermentation. Subsequently Hajna modified the medium by incorporating glycerol as additional growth factor to improve the fermentation ability of Enterococci. Also in the modified medium the concentration of the indicator dye i.e. bromocresol purple was decreased to aid easier detection and colour change within 24 hours. This modified medium is referred to as B.A.G.G Broth Base (Buffered Azide Glucose Glycerol Broth Base). The test sample can be directly inoculated into the medium. Depending upon the quantity of the test water sample, either single strength or double strength medium can be used. Presumptive faecal streptococci contained in B.A.G.G. Broth Base should be further tested for confirmation.