Guide-itTM CRISPR/Cas9 System (Green)

Description

The Guide-it CRISPR/Cas9 System Green is a complete system for cloning and expression of a target single guide RNA sgRNA for mammalian gene modification studies using CRISPR/Cas9 technology. The pGuide-it-ZsGreen1 Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein ZsGreen1. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and ZsGreen1 are coexpressed from a bidirectional CMV promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back The CRISPR/Cas9 system, a simple, RNA-programmable method to mediate genome editing in mammalian cells.The CRISPR/Cas9 system relies on a single guide RNA sgRNA directing the Cas9 endonuclease to induce a double strand break at a specific target sequence three base-pairs upstream of a PAM sequence in genomic DNA. This DNA cleavage can be repaired in one of two ways: 1 nonhomologous end joining, NHEJ resulting in gene knockout due to error-prone repair orange , or 2 homology-directed repair HDR , resulting in gene knockin due to the presence of a homologous repair template purple . Back Back