H2-I-A/I-E Antibody (M5/114): sc-23940

Description

H2-I-A/I-E Antibody M5/114 is a rat monoclonal IgG 2b H2-I-A/I-E antibody provided at 200 Amug/ml raised against activated C57BL/6 mouse spleen cells H2-I-A/I-E Antibody M5/114 is recommended for detection of I-A b , I-A d , I-A q , I-E d and I-E k MHC class II alloantigens of mouse origin by IF and FCM; non cross-reactive with I-A f , I-A k or I-A s ; detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes from mice carrying the H-2 b , H-2 d , H-2 q , H-2 p , H-2 r and H-2 u haplotypes, but not mice carrying the H-2 s or H-2 f Anti-H2-I-A/I-E Antibody M5/114 is available conjugated to agarose for IP; HRP for WB, IHC P and ELISA; and to either phycoerythrin or FITC for IF, IHC P and FCM also available conjugated to Alexa Fluor A 488 , Alexa Fluor A 546 , Alexa Fluor A 594 or Alexa Fluor A 647 for WB RGB , IF, IHC P and FCM, and for use with RGB fluorescent imaging systems, such as iBrighta FL1000, FluorChema, Typhoon, Azure and other comparable systems also available conjugated to Alexa Fluor A 680 or Alexa Fluor A 790 for WB NIR , IF and FCM; for use with Near-Infrared NIR detection systems, such as LI-COR A Odyssey A , iBrighta FL1000, FluorChema, Typhoon, Azure and other comparable systems Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 Amug sample of H2-I-A/I-E M5/114 : sc-23940 . At present, we have not yet completed the identification of the preferred secondary detection reagent s for H2-I-A/I-E Antibody M5/114 . This work is in progress.