HEK 293 HCP ELISA Kit, 3G

Description

Viral vector production in HEK 293 cells is a widely used procedure for obtaining sufficient quantities of a desired virus. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins HCPs from HEK 293 cells. The HEK 293 HCP ELISA Kit, 3G F650S can be used as a process development tool to monitor the removal of host cell impurities as well as in routine product release testing. The polyclonal antibodies used in this kit have been generated against and affinity-purified using a mild lysate of HEK 293 cells to obtain HCPs typically encountered in initial product recovery steps. The antibodies have broad coverage of 90 % to HEK 293 HCPs as determined by Antibody Affinity Extraction with 2D PAGE/Silver Stain and LC-MS. This HCP ELISA kit provides the specificity and sensitivity LOD 0.35 ng/ml, LLOQ 2 ng/ml to detect HEK293 HCPs with reproducibility that supports regulatory compliance. Please use Sample Diluent, Cat. No. I700 , with these kits. Note: The original pool of anti-HEK 293 HCP antibody has been depleted by Dec 31, 2021. The new Resupply 1 antibodies match the original F650S kit performance as close as possible. Kits containing the new resupply antibodies maintain the same catalog number, F650S, and are identified by the lot numbers starting with characters R1. Both Cygnus F650S HEK 293 HCP ELISA Kits, 3G, Original and Resupply 1, have gained global regulatory approvals for multiple commercially available gene and cell therapies.