HEPES Solution 1M in normal saline

Description

HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37AC and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which take place due to cellular metabolism. Unlike the bicarbonate buffers which require CO2, HEPES containing media do not need CO2 atmosphere for buffering. HEPES is generally used at a concentration of 10mM to 25mM for cell culture application. TCL190 is 1M HEPES solution prepared in sterile filtered saline solution.