HiPurA PCR Product Purification Kit

Description

The HiPurAA PCR Product Purification Kit provides a simple and rapid method to purify single or double stranded DNA from PCR and other enzymatic reactions. Fragments ranging from 100 bpa10 kb can be purified from primers, nucleotides, polymerases and salts by the use of silica binding in a microspin format that eliminates the need for expensive resins, alcohol precipitation and hazardous organic compounds such as phenol and chloroform. DNA binds specifically to the silicaagel membrane while contaminants such as free nucleotides, primer dimers upto 50 bp proteins, MgCl2, mineral oil etc. pass through and are completely removed in wash step. The clean, concentrated DNA is eluted in a small volume of low salt buffer. The purified products are ready to be used in cloning, sequencing and microarray analysis. The PCR product recovery is 80a95 % depending on the size of DNA.