HKSOX-1

Description

HKSOX-1 and its derivatives HKSOX-1r and HKSOX-1m are novel fluorescent probes designed for highly sensitive and selective detection of the superoxide anion radical O2AAasAasAa? in cellular environments. These probes utilize an aryl trifluoromethanesulfonate group that undergoes O2AAasAasAa?-mediated cleavage, releasing a free phenol and emitting fluorescence. They demonstrate excellent specificity and sensitivity across various pH ranges, withstand interference from strong oxidants and reductants typical in cellular contexts. HKSOX-1r, optimized for cellular retention, has been effectively employed in diverse assays including confocal imaging, flow cytometry, and zebrafish embryo studies, highlighting its utility in investigating O2AAasAasAa? roles in inflammation, mitochondrial stress, and other physiological processes.