Human Bone Marrow Marathon-Ready cDNA

Description

Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5 and 3 Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-mul Marathon RACE reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA.RACE PCR was performed. The template for Lanes 13 was Marathon-Ready Human Placenta cDNA Cat. # 639311 ; the template for Lanes 4 & 5 was Marathon-Ready Human Skeletal Muscle cDNA Cat. # 639313 . Lane 1: 5-RACE product for TFR cDNA. Lane 2: 3-RACE product for TFR cDNA. Lane 3: full-length TFR cDNA 5.1 kb generated by end-to-end amplification using 5 and 3 TFR primers. Lane 4: nearly full-length IGFR2 cDNA 8.8 kb amplified with 5 IGFR2 and 3 IGFR2 primers. Lane 5: full-length IGFR1 cDNA 5.0 kb amplified with primers 5-IGFR and 3-IGFR. Lane M: 1-kb DNA size ladder. Back Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs.RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart Cat. # 639304 , Mouse Heart Cat. # 639404 , and Rat Heart Cat. # 639416 cDNAs. Lane M: 1-kb DNA size ladder. Required Products This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5 and 3 Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions. Back Marathon cDNA amplification of abundant actin, 1.9 kb and moderately rare TFR, 5.1 kb transcripts.The 5- and 3-RACE reactions for actin and TFR were performed with adaptor-ligated ds cDNA made from 1 mug of human placental poly A+RNA and amplified for 25 PCR cycles. Full-length cDNAs were end-to-end PCRamplified according to the User Manual. Lane 1: 1.2-kb actin 5-RACE product. Lane 2: 1.3-kb actin 3-RACE product. Lane 3: full-length 1.9-kb actin cDNA. Lane 4: 2.6- kb TFR 5-RACE product. Lane 5: 2.9-kb TFR 3-RACE product. Lane 6: full-length 5.1-kb TFR cDNA. Lane M: 1-kb DNA size ladder. Back