Human CRF (Corticotropin Releasing Factor) ELISA Kit

Description

Synonym: CRH; Corticotropin Releasing Hormone; Corticoliberin. This Human CRF (Corticotropin Releasing Factor) ELISA Kit applies to the in vitro quantitative determination of Human CRF concentrations in serum, plasma and other biological fluids. Type:Competitive, Assay time:1.5h, Detection type:Colormetric, Precision:CV<10%, Assay Range:12.35-1000pg/mL, Sensitivity:5.05pg/mL, Recovery:80%-120%, Cross reactivity:The kit detected Human CRF in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human CRF. During the reaction, Human CRF in the sample or standard competes with a fixed amount of Human CRF on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CRF. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human CRF in tested samples can be calculated by comparing the OD of the samples to the standard curve.