iDimerizeTM Inducible Heterodimer System

Description

The iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells via a membrane-permeant compound. The system includes the iDimerize Inducible Heterodimer Vector Set 1, which contains three mammalian expression vectors encoding fusion tags that can be easily added to your proteins of interest. The activity and localization of the resulting chimeric proteins can be controlled by the addition of a small molecule to the cell medium. The system also includes the membrane-permeant compound, A/C Heterodimerizer, required for heterodimerization, and two linear selection markers for hygromycin and puromycin resistance. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back How does iDimerize technology work? A chemical inducer of dimerization, or dimerizer, is a cell-permeant organic small molecule with two separate motifs that each bind with high affinity to a specific protein module Dmr domain fused onto the protein s of interest.Addition of the dimerizer brings the chimeric protein subunits into very close proximity to each other, mimicking the activation of the cellular event that dimerization of interest controls. B/B Homodimerizer induces self-association of two copies of the same protein whereas A/C Heterodimerizer induces association of two different proteins. Conversely, a reverse dimerizer ligand D/D Solubilizer will bind to and dissociate a protein that aggregates in its absence. Back