Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1)

Description

Mesenchymal stromal cells are observed to have immunosuppressive effects on lymphocytes. The Immortalized Canine Bone Marrow Mesenchymal Stromal Cells DS1 cells were generated from mononuclear cells derived from canine bone marrow. The cells were immortalized via transduction of a retrovirus containing the human papilloma virus E6/E7 genes. Phenotypic characterization of the Immortalized Canine Bone Marrow Mesenchymal Stromal Cells DS1 cells via RT-PCR found that the cell line was weakly positive for CD10, CD29, CD106, moderately positive for CD73 and CD105, and strongly positive for CD90. The Immortalized Canine Bone Marrow Mesenchymal Stromal Cells DS1 cells are applicable studies to investigate their immunosuppressive effects, especially in inflammatory and immune disease models.