Immortalized Human Cranial Suture Progenitor Cells (iSuPs)

Description

This cell line was generated using primary SuPs derived from patients with craniosynostosis, with retroviruses carrying SV40 T antigen, flanked by FRT or loxP sites, using hygromycin B as a selection marker. These cells continuously proliferate in culture, express a large number of mesenchymal stem cell markers, and retain osteogenic and adipogenic potential upon addition of BMP9 to the media. The immortalization can be reversed by expression of FLP recombinase to excise the SV40 T gene. iSuPs are a valuable tool for studying craniosynostosis, intramembranous ossification, and adipogenesis, as well as potential therapeutic drugs for associated pathologies.