Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1))

Description

In aldosterone-sensitive distal nephron ASDN , mineralocorticoid receptor MR and glucocorticoid receptor GR activation regulate ionic and water transports through the epithelial sodium channel ENaC and the Na+/ K+ pump. The Immortalized Mouse Cortical Collecting Duct Cell Line is a spontaneously transformed Liddle mouse cell line that expresses significant level of functionally active MR and GR, as well as active 11beta-hydroxysteroid dehydrogenase type 2 11beta-HSD2 enzyme. In addition, this cell line responses to physiological concentrations K 1/2 = 0.3 nM of aldosterone, making it useful for binding equilibrium assays and studies on sodium transport response to various mineralocorticoid and glucocorticoid agonists.