Immortalized Mouse Neuronal Cells (M4b)

Description

The Immortalized Mouse Neuronal Cells M4b were derived from the spinal cord of a fetal mouse after 12-16 days of gestation. The fetal mouse was bred from double heterozygous Rb 2H/RB 32 Lub males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Mouse Neuronal Cells M4b . In addition, the intracellular Ca2+ response after neurotransmitter stimulation was rapid and transient as expected. These cells are useful in studies of neuronal cell biology, viral infections, and neuronal cytotoxicity. Additionally, T0674 can be used as a control for the Immortalized Trisomy 16 Mouse Neuronal Cells MTh Cat. No. T0675 , which is a mouse model of Down Syndrome.