Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)

Description

IMPE is a conditionally immortalized mouse pancreas epithelial cell line isolated from the mouse harboring thermolabile mutation tsA58 under the control of an interferon IFN -gamma-inducible H-2Kb promoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN gamma at a temperature permissive 33C . At a non-permissive temperature 37C-39C or when cultured without IFN gamma over 3 passages, the cells cease to proliferate. The IMPE has been shown to produce insulin after transfection with pdx1 and culture in the presence of glucagon-like peptide 1 GLP1 .