Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40

Description

The Immortalized Rat Conjunctival Epithelial Cells CJ4.3 were derived from primary conjunctival epithelial cells from Fischer 344 rats. The primary cells were immortalized with pSV neo and transfected with SV40 large T antigen. The immortalized cells display epithelial morphology and express the conjunctiva-specific cytokeratin 4, the goblet cell-specific cytokeratin 7 and were negative for the corneal epithelial cell-specific cytokeratin 12. The cells are useful as models for studying the conjunctival epithelial barrier and also in developing strategies for delivering macromolecular antigens across this barrier to underlying components of the immune system. Immunofluorescence confocal microscopy demonstrated cytokeratin expression.