Immortalized Trisomy 16 Mouse Neuronal Cells (MTh)

Description

The Immortalized Trisomy 16 Mouse Neuronal Cells MTh were derived from the spinal cord of a trisomy 16 fetal mouse after 12-16 days of gestation. Trisomy 16 in mice is an animal model for trisomy 21, or Down Syndrome, in humans. Since trisomy 16 animals die in utero , the immortalized Trisomy 16 Mouse Neuronal Cells MTh offers an immortalized in vitro model. The fetal mouse was bred from double heterozygous Rb 2H/RB 32 Lub males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. RT-PCR found an overexpression of enzyme SOD-1, encoded on mouse chromosome 16 and human chromosome 21, and confirmed the trisomy status of the cell line. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Trisomy 16 Mouse Neuronal Cells MTh . Upon comparison to Immortalized Mouse Neuronal Cells M4b Cat. No. T0674 , the Immortalized Trisomy 16 Mouse Neuronal Cells MTh were found to have a greater basal Ca2+ and a greater sensitivity to neurotransmitters. No significant difference in choline uptake was observed between the two cell lines. The Immortalized Trisomy 16 Mouse Neuronal Cells MTh can be used as an in vitro model to study neuronal biology and physiology related to Down Syndrome.