In-Fusion Snap Assembly Value Bundle

Description

In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: - A NucleoSpin Gel and PCR Clean-Up kit: This kit is suitable for gel extraction as well as PCR purification. Kits are provided with individual purification columns. - Stellar Competent Cells: High-efficiency competent cells are essential to the success of In-Fusion Cloning. AnE. coliHST08 strain is included that provides high transformation efficiency greater than 5 x 108 cfu/mug and complements the efficiency of all In-Fusion Snap Assembly kits. Cells are provided in 100-mul aliquots in individual tubes. - PrimeSTAR Max DNA Polymerase: This convenient 2X liquid master mix offers exceptionally accurate, efficient, and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid setup of PCR reactions, and facilitates successful cloning. The polymerase master mix is provided in 625-mul aliquots. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.A single 3.8-kb insert Panel A or a 34.2-kb adenovirus insert Panel B was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing for the 3.8-kb insert or colony PCR for the adenovirus insert to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi. Back The In-Fusion cloning protocol. Back