IP6K1(-/-) Stable HCT116 Cell Line

Name: IP6K1(-/-) Stable HCT116 Cell Line
Brand: abm good

T6349 · 1 x 106 cells / 1.0 mL

abm good · Cat: T6349

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Catalog No: T6349

Category: Cellular Materials

Pack Size: 1 x 106 cells / 1.0 mL

Brand: abm good

Description

To study the role that PP-IPs play in mammalian phosphate homeostasis researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs. IP6K1-/- line did not have significantly less IP7 than WT wildtype . IP6K3 is expressed mainly in muscle cells and the cell lines tested were not muscle-derived and contained no or a very low amount of IP6K3 mRNA. Hence they chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only and has easily detectable levels of IP7 and IP8.