iQue Human T Cell Exhaustion Kit

Description

The Human T Cell Exhaustion Cell and Cytokine Profiling Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages: Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye. Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells. The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3. Optimized workflow enables the measurement of cytokines secreted by activated T cells IFNgamma and TNF in the same assay well. The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired included with the kit . Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.