iQue Human T Cell Killing Kit

Description

The Human T Cell Mediated Killing Kit was designed for ease of use in multiplexing cell phenotype and function markers along with bead-based, secreted protein profile measurements in the same assay. This one wash assay requires minimal hands on time to provide the following biological insights: Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye. Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD8+ T cytotoxic cells, and CD3+ CD8- T helper cells. The panel also includes 2 different T cell functional markers, CD25 and PD-1. Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNgamma and pro-apoptotic serine protease Granzyme B in the same assay well. Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.