iQue Mouse IgG Type and Titer

Description

The Mouse IgG Type and Titer kit is a multiplexed, competition formatted assay to identify and quantify mouse IgG1, IgG2a, IgG2b, and IgG3. It is a no wash, no sample dilution assay that measures both cells and beads and provides these significant advantages over traditional methods: IgG isotype identification: Guides the decision-making process by identifying monoclonal wells, thus reducing the number of cell sub-cloning steps while facilitating isotype-specific DNA primer sets rather than degenerate primers for cloning or sequencing of antibody variable regions. Precise total and isotype specific IgG quantification: Downstream confirmatory or functional assays benefit from normalizing the IgG concentration for all clones. Simultaneous measurement of cell count and cell viability: Identifies the cells healthy enough for the RNA extraction needed for PCR-mediated antibody cloning. Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.