LA PCRTM Kit, Version 2.1

Description

This kit includes all the reagents necessary for high-fidelity amplification of long DNA templates, with routine extension to 30 kb up to 48 kb is possible with some templates . The kit also contains a control template and primer sets. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Amplification of human genomic DNA with TakaraLA TaqDNA Polymerase.Purified human genomic DNA 500 ng in a 50 muL reaction was used as a template for PCR amplification of regions of the beta-globin locus and the TPA gene with TakaraLA Taqpolymerase. The sizes of the amplified products were 17.5 kb lane 1 , 21.5 kb lane 2 , and 27 kb lane 3 . Lane M contains High MW Markers Fisher Scientific . Back Amplification ofE. coligenomic DNA regions with TakaraLA Taqpolymerase.DNA fragments up to 38 kb were successfully amplified with the LA PCR Kit Version 2.1, facilitating studies using P1 and cosmid clones. Back