LEGIONELLA GROWTH SUPPLEMENT (BCYE)

Description

Warm up the refrigerated supplement to 45-50?C, shake well to form uniform suspension. Avoid frothing. Aseptically add to 440 ml sterile, molten, cooled (45-50?C) TM 1218 – LEGIONELLA AGAR BASE / TM 2156 – LEGIONELLA AGAR BASE W/O CHARCOAL. If desired aseptically add rehydrated contents of 1 vial of Legionella (GVPC) Selective Supplement. Mix well and pour into sterile petri plates. The final pH of the medium will be 6.9 ? 0.2. In case of non-incorporation of Legionella (GVPC) Selective Supplement, add aseptically 10 ml sterile distilled water to bring the total volume to 500 ml medium. Note: On storage at 2 to 8?C; due to combination of ingredients, slight fine precipitate may develop which may be evenly distributed before addition to medium.