Lenti-XTM 293T Cell Line

Description

The Lenti-X 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of viral protein expression. When transfected with Lenti-X Packaging Single Shots and a lentiviral vector, these cells are capable of producing lentiviral titers as high as >108ifu/ml, as determined by flow cytometry. The cell line also constitutively expresses the simian virus 40 SV40 large T antigen. Back High-titer lentivirus production.Lenti-X 293T cells were transduced with the indicated volumes mul of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet. Back Back Back Transduction of neural progenitor cells by Lenti-X lentivirus.Recombinant lentivirus for expressing ZsGreen1 was produced using Lenti-X virus and used to transduce normal human neural progenitor cells. A single transduced cell is shown under phase contrast microscopy Panel A and fluorescence microscopy Panel B . Back 293T cell line for higher titers.We used our fourth-generation lentiviral packaging system and one of our pLVX-lentiviral vectors to compare the virus production of the Lenti-X 293T Cell Line to that of two other commonly used HEK 293-based cell lines. Lenti-X 293T cells clearly outperformed the other cell linesproducing over 6X more virus than 293FT cells and up to 30X more virus than the parental HEK 293 cell line.