Lenti-XTM Accelerator

Description

Lenti-X Accelerator is a magnetic bead-based technology designed to accelerate lentiviral transduction experiments. Traditional lentiviral transductions require overnight incubation of your cells and virus with Polybrene, but Lenti-X Accelerator allows you to complete this process in only 30 minutes and avoid using Polybrene altogether. Charged magnetic beads bind to virus particles from your lentiviral packaging supernatant and are pulled into contact with your cells in the presence of a magnetic field. A Magnetic Separator for Cell Culture Cat. No. 631255 is available separately, or supplied together with the Lenti-X Accelerator reagent in the Lenti-X Accelerator Starter Kit Cat. No. 631254 . Lenti-X Accelerator is excellent for transducing lentivirus, MMLV retrovirus, and MSCV retrovirus preparations. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Transduction with Lenti-X Accelerator versus Polybrene.Placing virus-bound magnetic beads in a magnetic field greatly increases the localized virus concentration at the surface of the cell monolayer. This reduces the transduction time to just 5 min after a 20 min preincubation to bind the beads to the viruscompared to transduction overnight with Polybrene. Accelerated transduction also limits exposure of your sensitive target cells to viral supernatant. Back Lenti-X Accelerator 25 min is faster than Polybrene 16 h for lentiviral transduction.A ZsGreen1-expressing Lenti-X vector was used to transduce HT1080 cells. Lenti-X Accelerator beads 8 mul were preincubated for 20 min at room temperature with 200 mul approx. 1x106total IFU of viral supernatant and applied to HT1080 cells on a Magnetic Separator for 5 min. HT1080 cells were also transduced with the same amount of virus in the presence of 6 mug/ml Polybrene, for 5 min or overnight. After the cultures were grown for an additional 72 hr at 37C, the number of transduced cells was determined by flow cytometry Back Back