Lenti-XTM Expression System

Description

The Lenti-X Expression System enables you to produce exceptionally high titers of recombinant lentivirus for expression of any cDNA in any cell type susceptible to lentivirus transduction. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious lentivirus particles. Lenti-X Packaging Single Shots, which contain lyophilized Lenti-X packaging plasmids and transfection reagent, are used for efficient introduction of the packaging mix and the pLVX-Puro Vector into 293T cells with very high efficiency. The concerted effects of these components allow 293T cells to produce high-titer replication incompetent lentiviral supernatants for a safe transduction of virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Fourth-generation lentiviral packaging system.A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection. Back Back Back High-titer lentivirus production.Lenti-X 293T cells were transduced with the indicated volumes mul of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.