Lenti-XTM p24 Rapid Titer Kit

Description

The Lenti-X p24 Rapid Titer Kit allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a standard ELISA. The wells of the included microtiter plate 12 x 8-well strips are coated with anti-HIV-1 p24 capture antibody, which binds the p24 an HIV-1 core protein in your test samples. Specifically-bound p24 is detected in a typical sandwich ELISA format using a biotinylated anti-p24 secondary antibody, a streptavidin-HRP conjugate, and a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lentiviral titration by p24 ELISA or Lenti-X GoStix.The p24 protein is located in the lentiviral capsid and is one of four proteins encoded by the HIV-1gaggene. The amount of p24 present in packaging cell supernatants can be used to measure lentiviral titer. Back Lenti-X p24 Rapid Titer Kit to measure lentivirus titer.Lentiviral supernatant is bound to anti-p24-coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a color reagent.