Lenti-XTM p24 Rapid Titer Kit (Single Wash)

Description

The Lenti-X p24 Rapid Titer Kit Single Wash allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a fast and easy ELISA-based protocol. The test samples are mixed with anti-p24 antibody, HRP-conjugated secondary antibody, and lysis buffer. This mix is then added to the wells of the included microtiter plate 12 x 8-well strips which are coated with anti-HIV-1 p24 capture antibody and binds the p24 an HIV-1 core protein in your test samples. After a single wash step, the specifically-bound p24 in the sample is detected with a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lentiviral titration by p24 ELISA or Lenti-X GoStix.The p24 protein is located in the lentiviral capsid and is one of four proteins encoded by the HIV-1gaggene. The amount of p24 present in packaging cell supernatants can be used to measure lentiviral titer. Back Workflow.Lentiviral p24 core protein in packaging cell supernatants is bound to wells of a microtiter plate coated with p24 capture antibody. The presence of bound p24 in the wells is detected using an anti-p24 antibody, HRP-conjugated secondary antibody, and a color-producing substrate. Quantitation is performed by comparing test samples to a p24 standard curve. Back Schematic comparing the workflow of the Lenti-X p24 Rapid Titer Kit Single Wash Cat #631476 to Lenti-X p24 Rapid Titer Kit Cat #632200 .The Lenti-X p24 Rapid Titer Kit Single Wash protocol involves fewer steps and only a single wash step. Back Lenti-X p24 Rapid Titer Kit Single Wash standard curve.p24 standard dilutions were prepared and assayed as described in the user manual. The table shows the assay results for the p24 standard dilutions and the graph was generated from the values in the table.