

631235 · 200 Rxns
Takara · Cat: 631235
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Manufacturer product page
takarabio.com/products/gene-function/viral-transduction/lent...
Description
This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle Ct of an appropriate dilution of purified viral RNA to sample curve Ctvalues that are derived from a calibrated, serially diluted control viral RNA template. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods.Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day. Back







