Lenti-XTM T-Cell Transduction Sponge

Description

The Lenti-X T-Cell Transduction Sponge expedites and streamlines lentiviral transduction of T cells, bypassing the need for prior activation or spinoculation with chemical enhancers. This innovative approach employs a macroporous alginate matrix infused with an optimized blend of rhIL-2 100 IU and anti-human CD3 & CD28 antibodies. This combination with the sponge not only activates T cells but also enhances lentiviral transduction by fostering the co-localization of activated T cells and lentivirus particles. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Visual overview of the Lenti-X T-Cell Transduction Sponge workflow. Back Lenti-X T-Cell Transduction Sponge.Panel A.Sponges 12 in the crush-resistant blister packaging and magnified view of the macroporous cell structure of the alginate.Panel B.Illustrated visualization of colocalization of T cells and virus promoted by the sponge structure. Back Effective T-cell transduction with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated MOI and added to the Lenti-X T-Cell Transduction Sponge T-cell sponge . Spinoculated samples were activated with CD3/CD28 ImmunoCult reagent for two days, then exposed to the lentivirus with polybrene 8 mug/ml and centrifuged at 1,500gfor 90 min Spinoculation . Transduction efficiency was assessed by FACS analysis for ZsGreen1 expression at the indicated time points. Displayed percentages show GFP+ expression at seven days. Back High T-cell viability after transduction with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated infection MOIs and added to the Lenti-X T-cell Transduction Sponge T-cell sponge . Spinoculated samples were activated with CD3/CD28 ImmunoCult reagent for two days, then exposed to the lentivirus with polybrene 8 mug/ml and centrifuged at 1,500gfor 90 min Spinoculation . Viability was assessed at indicated timepoints post-transduction by 7-AAD staining and FACS analysis. Back Efficient T-cell activation with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated multiplicities of infection MOIs and added to the Lenti-X T-cell Transduction Sponge T-cell sponge or treated with CD3/CD28 Immunocult reagent Conjugate . Unstimulated cells were used as a control Unstim . After 48 hr, treated cells were assayed for CD69 expression by FACS analysis.