Linear Puromycin Marker

Description

Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector including Tet System vectors for generating stable transfectants using CalPhostransfection reagent or a liposome-mediated transfection protocol. Back Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker. Back