LKB1 Stable Knockdown Cell Line (H522-408)

Description

This cell line features a LKB1 Stable Knockdown Using Lentiviral Short Hairpin RNA. To select for the NSCLC cells that were stably expressing shRNA constructs, cells were plated at subconfluent densities and infected with a cocktail of 1 mL of virus-containing medium, 3 mL of regular medium, and 8 ug/mL polybrene. Selection with 0.5 to 2 ug/mL of puromycin was started 48 h after lentivirus infection. After several weeks of selection 2 wk for H1299 or 4 wk for H1650, H522 , monolayers of stably infected pooled clones were harvested for use and cryopreserved.