LURIA AGAR

Description

Luria Agar is prepared as described by Lennox for cultivation and maintenance of recombinant strains of Escherichia coli. The media is generally used for molecular and genetic studies, because of its nutritive capacity and simple composition, which can be easily altered as per specific requirements. The medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains which are generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis and are further modified by specific mutation to create auxotrophic strains that are unable to grow on nutritionally deficient media.