LURIA BROTH

Description

Luria Broth is one of the many modifications, suggested by different authors, of the original formulation of Luria. This medium is generally used for molecular and genetic studies, because of its nutritive capacity and simple composition, which can be easily altered as per specific requirements. Luria Broth is the modification of the original formulation of Luria, as described by Lennox. Luria Broth contains half the concentration of sodium chloride than in Luria Broth, Miller. Therefore as per choice, the sodium chloride concentration can be altered. Luria Broth is used for the cultivation and maintenance of recombinant strains of E. coli, originally derived from E. coli strain K12, deficient in B vitamin production. These stains are specifically mutated to create an auxotrophic strain, unable to grow on a nutritionally deficient medium.