Lyo-Ready One Step PrimeScriptTM III RT-qPCR Mix

Description

Lyo-Ready One Step PrimeScript III RT-qPCR Mix is designed to be directly incorporated into a lyophilization process. It contains the same robust chemistry as One Step PrimeScript III RT-PCR Mix Cat # RR600A & RR600B but with an excipient for lyophilization. No additional excipients are required. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Linear dynamic range of Lyo-Ready One Step PrimeScript III RT-qPCR Mix and Lyophilized One Step PrimeScript III RT-qPCR Mix compared to the One Step PrimeScript III RT-PCR Kit.Three replicates of a 7-fold dilution series of human RNA were tested using human GAPDH primers/probes with PanelA the One Step PrimeScript III RT-PCR Kit Cat. #RR600 , PanelB Lyo-Ready One Step PrimeScript III RT-qPCR Mix, or Panel C Lyophilized One Step PrimeScript III RT-qPCR Mix. For each RT-qPCR Mix, amplification curves and standard curve plots with PCR efficiency and R2values are shown. Lyo-ready and lyophilized one-step PrimeScript III demonstrate the same consistent amplification across all dilution series points as the One Step PrimeScript III RT-PCR Kit. Back Performance of Lyo-Ready One Step PrimeScript III RT-qPCR Mix in a 5-plex assay.A 5-fold dilution series of human RNA was tested in a 5-plex assay using Lyo-Ready One Step PrimeScript III RT-qPCR Mix and primer/probe sets for the following human genes: ACTB, GAPDH, HRPT1, PPA, and RNAseP. The standard curve plot, PCR efficiency, and R2value for each gene target in the 5-plex assay are shown. Lyo-Ready One Step PrimeScript III RT-qPCR Mix maintained comparable performance for all 5 genes in the multiplex assay, as demonstrated by the standard curve plots, qPCR efficiencies, and R2values for each individual gene. Back