m-IgGλ BP-CFL 680: sc-516194

Description

mouse IgG lambda binding protein m-IgGI BP conjugated to CruzFluora 680 CFL 680 supplied at 200 Amug in 0.5 ml volume CruzFluora 680 CFL 680 is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor A 680, offering comparable photostability and the ability to resist protein quenching Excitation of 683 nm, Emission of 700 nm Suitable for use with Near-Infrared NIR imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting WB NIR , Immunofluorescence IF and Flow Cytometry FCM Suitable for binding to most, but not all, mouse IgGI light chain immunoglobulins; not suitable for use with mouse monoclonal IgGIo light chain antibodies Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies For mouse IgGIo immunoglobulins, comprising approximately 98 % of mouse monoclonal antibodies we recommend m-IgGIo BP-CFL 680 sc-516180 For optimal results, use low fluorescence PVDF membranes, such as Immobilon A -FL PVDF, and UltraCruz A Blocking Reagent sc-516214 for all blocking, primary antibody and mouse IgG binding protein incubations. Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.