MacCONKEY BROTH W/ BCP (DOUBLE STRENGTH)

Description

MacConkey Broth Purple (Double Strength) w/ BCP is a modification of MacConkey Medium. Childs and Allen demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. Liquid specimens are directly inoculated while solids have to be homogenized in appropriate diluents such as physiological saline, phosphate buffers, etc. The inoculation must be effected at 10% v/v in Durhams tubes. If the inoculum is greater than 1 ml, it is necessary to use the medium at double strength, inoculating equal volumes of specimen and medium.