Make Your Own "Mate & PlateTM" Library System

Description

Mate & Plate Libraries are by far the easiest libraries to screen for protein-protein interactions using a GAL4 yeast two-hybrid system. Several Mate & Plate Libraries are available ready-made from Takara Bio. For libraries that are N/A, this system provides the necessary components and a simple, highly efficient method to make your own Mate & Plate Library using SMART technology and the highly efficient homologous recombination machinery ofSaccharomyces cerevisiae. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back Back Required Products This kit is based on Takaras Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 100 PCR reactions of 50 mul each. Back Back Back Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain AD fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker. Back Back Back Back This kit is based on Takaras Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 mul each. Back Back Back