Mal-Cyclohexyl-PEG3-Biotin

Description

Mal-Cyclohexyl-PEG3-Biotin is a PEG linker containing a maleimide moiety and a biotin group. Maleimides react specifically with sulfhydryl groups to form a stable thioether linkage when the pH is between 6.5 and 7.5. The biotinylation can react with amine molecules in the presence of activator EDC or HATU. PEG attached to the biotin gives an extended spacer arm that permits the biotin to reach into the binding pocket of the protein. The PEG moiety also increases solubility of Biotin-PEG conjugates considerably.