Marathon cDNA Amplification Kit

Description

This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5 and 3 Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Marathon cDNA amplification of abundant actin, 1.9 kb and moderately rare TFR, 5.1 kb transcripts.The 5- and 3-RACE reactions for actin and TFR were performed with adaptor-ligated ds cDNA made from 1 mug of human placental poly A+RNA and amplified for 25 PCR cycles. Full-length cDNAs were end-to-end PCRamplified according to the User Manual. Lane 1: 1.2-kb actin 5-RACE product. Lane 2: 1.3-kb actin 3-RACE product. Lane 3: full-length 1.9-kb actin cDNA. Lane 4: 2.6- kb TFR 5-RACE product. Lane 5: 2.9-kb TFR 3-RACE product. Lane 6: full-length 5.1-kb TFR cDNA. Lane M: 1-kb DNA size ladder. Back